Firefighters deployed nine planes and a helicopter to fight the blaze.
The fire, which broke out at 10 a.m. local time (0800 GMT) on Saturday, spread on two fronts, one towards the village of Vrisa and the other towards the interior of Vatera.
The mayor of Lesbos West, Taxiarchis Verros, ordered the evacuation of the bustling resort town as a precaution, on the advice of firefighters, Greek news agency ANA reported.
The mayor did not specify the number of people evacuated, but several buses and small boats took part in the operation.
At least two houses were ravaged by the flames, according to state television ERT.
On Saturday, firefighters were also carrying out their third day of fighting a violent fire in Dadia National Park, the country's largest Natura 2000 site, known for its colony of vultures, in the Evros region, in the northeast of Greece.
Firefighter spokesman Yiannis Artopoios said on Saturday afternoon that the fire was "exceptionally difficult", but it "looks better in the northeast".
A total of 320 firefighters and 68 devices have been deployed since Friday evening, as well as 13 ground teams, and a very large number of volunteer firefighters.
In addition, six water bomber helicopters and nine helicopters operate in the sky.
However, dense smoke and a lack of wind, combined with high humidity overnight, hampered firefighting efforts in the air.
On Wednesday, a wildfire in the mountains near Athens damaged homes and forced several hundred people to evacuate.
In 2018, Greece experienced its worst disaster caused by a forest fire: in Mati, a coastal village located about 30 km from Athens, a fire killed 102 people, a few kilometers from the affected area on Wednesday.
A tribute to the victims was paid on Saturday in the town of Mati.
Last year, a heat wave and forest fires destroyed 103,000 hectares and killed three people in Greece.
The proliferation of extreme weather events is a direct consequence of global warming according to scientists, with greenhouse gas emissions increasing in intensity, duration and frequency.
